![]() ![]() Package to guide you through E-Phys experiments, incl. Wizard for fast and reliable setup of FCS and FLCS experiments and full control of F(L)CS data acquisition. Wizard for fast and reliable setup of FLIM experiments and full control of FLIM data acquisition. Restriction of detection to a certain time window removes unwanted signal from autofluorescence or backscattering with HyD detectors. Tool to compensate within a z-stack drop of fluorescence intensity that occurs deeper inside your sample with laser power and/or detector gain. Allows Lambda square fluorescence mapping. Imaging method that acquires the full excitation-emission spectrum in each image pixel using a tunable laser and spectral detectors. Imaging method that acquires the emission spectrum in each image pixel using spectral detectors. Includes powerful trigger capabilities such as sending a trigger at a specific channel or position.Ĭonfocal users can select different dyes and LAS X sets up all hardware components accordingly. Incorporate triggers into your time lapse experiments. Send triggers and analog signals to peripherals. Versatile tool set for high content screening and automated microscopy. The step by step wizard guides users through setup, acquisition and quantification of a FRAP experiment Log the environmental data and monitor it during the experiment.Īllows system administrators to give different access levels to different LAS X user groups. Have full control of your experimental conditions with the LAS X Environmental Control module. Powerful wizard for FRET acquisition and analysis Histogram based colocalization and area measurements Interactive data recording allowing job-sequencing and online evaluation Module for online ratio measurement, online display of ratio graphs and ratio image Automatically distribute regions and positions to each well/chamber.Īllows the control of any third party camera supported by LAS X.Įffectively sums up only the in focus area of each image in a three dimensional image stack and creates a single EDOF image. Perform multi well and multi chamber experiments. Includes software autofocus.ĭefine multiple stage locations and revisit them as part of a time-lapse or Z stacking experiment.Ĭreate multiple regions of any shape for overview images and high resolution scans Protect from light.LAS X Core software, operates without the need for a dongle.Īllows the definition of up to 8 acquisition channels per experiment.ĭefine the duration and frequency of image capture for time lapse experiments.įocus positioning and acquisition of 3 dimensional data sets. Fulfills highest requirements on antibody validation: structure and function characterized.Strong avidity effect from bivalent form of Spot-VHH.Superior accessibility and labelling of epitopes in crowded cellular/organelle environments.Capture and detection tag without compromises in applications.Due to its small size, Spot-Label is optimal for effective labeling with minimal fluorophore displacement for super-resolution microscopy. Spot-Label is much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Immunofluorescence of Spot-tagged proteins with anti-Spot Nanobody conjugated to fluorescent dye. STED images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.Īnti-Spot-Tag VHH/ Nanobody conjugated to fluorescent dye for immunofluorescence, microscopy, and immunoblotting of Spot-tagged proteins Description ![]() Images were deconvolved with Huygens Professional (SVI). Pixel size: 21 x 21 nm z-Step size of z-Stacks: 0.16 μm. ![]() Gated STED images were acquired with a Leica TCS SP8 STED 3X microscope with pulsed White Light Laser excitation at 590 nm and pulsed depletion with a 775 nm laser. STED: IF of Spot-tagged Actin-Chromobody with Spot-Label Atto594 bivalent (1:1,000). STED images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich. ![]()
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